COMMUNITIES · IMAGES · CURRENTS

The most revealing processes often happen between layers.

Between Layers connects community ecology, biomedical imaging, and cellular electrophysiology to explore how researchers identify interfaces, measure structure, and interpret interactions across biological scales.

Independent educational resource

SECTION VIEW / THREE SCALES
SCALE LINECOMMUNITYSTRUCTURECELL
INTERFACE 01
INTERFACE 02
ROOT / COMMUNITY

How do interactions among plants, fungi, and environmental conditions shape ecological communities?

Competition, facilitation, symbiosis, traits, and environmental gradients contribute to community structure.

SLICE / IMAGE

How can anatomical structures become measurable through images?

Segmentation, registration, geometry, and image analysis turn visual information into quantitative descriptions.

MEMBRANE / CURRENT

How do ion channels shape electrical behavior across cell membranes?

Channel opening, ion selectivity, voltage, and pharmacology influence cellular electrical activity.

Interaction · Representation · Current · ScaleThese scientific domains are compared methodologically, not as equivalent biological mechanisms.

LOOK BETWEEN THE LAYERS

A boundary can separate structures—and also reveal how they interact.

Communities emerge through interaction.

Images depend on representation.

Membranes regulate exchange.

Interpretation depends on scale.

FOUR WAYS TO READ BIOLOGICAL STRUCTURE

Different layers require different evidence.

Explore how ecological communities, biomedical images, and cellular systems become observable through different research methods.

01ROOT

Community Structure

Explore how competition, facilitation, plant traits, environmental conditions, and species interactions contribute to the composition and diversity of ecological communities.

  • Community ecology
  • Biodiversity
  • Plant interactions
  • Environmental gradients
02NETWORK

Symbiotic Networks

Study relationships between plant roots and mycorrhizal fungi, including mutualistic interactions, fungal communities, plant distribution, and ecological assembly across local and global scales.

  • Mycorrhiza
  • Plant-fungal interactions
  • Mutualism
  • Community assembly
03SLICE

Biomedical Images

Examine how image processing, segmentation, registration, geometry, pattern recognition, and quantitative analysis can transform medical images into measurements of biological structure.

  • Medical imaging
  • Image analysis
  • Segmentation
  • Registration
04MEMBRANE

Cellular Currents

Explore membrane physiology, ion channels, cardiac electrical activity, channelopathies, pharmacology, and the movement of ions that contributes to cellular signaling.

  • Ion channels
  • Electrophysiology
  • Channelopathies
  • Membrane physiology

WHAT HAPPENS AT AN INTERFACE?

Interfaces are places where structure, exchange, and measurement meet.

INTERFACE 01

ROOT ↔ FUNGUS

How can below-ground symbiosis influence plant community structure?

PLANT TRAITSINTERFACEFUNGAL COMMUNITY

The interface is ecological and biological. It is shaped by species, environmental conditions, traits, and reciprocal exchange.

  • mycorrhiza
  • mutualism
  • roots
  • community assembly
  • biodiversity
INTERFACE 02

IMAGE ↔ ANATOMY

How does an image representation become a measurement of anatomy?

IMAGE DATAINTERPRETATIONQUANTITATIVE STRUCTURE

An image is a representation, not the biological structure itself. Analysis choices influence the measurements researchers obtain.

  • segmentation
  • registration
  • geometry
  • image processing
  • measurement
INTERFACE 03

MEMBRANE ↔ ION

How can selective ion movement alter cellular electrical activity?

CONCENTRATION / VOLTAGEION CHANNELCELLULAR CURRENT

Ion channels are membrane proteins whose properties influence ionic currents and cellular electrical behavior.

  • hERG
  • potassium
  • action potential
  • patch clamp
  • pharmacology

THE CROSS-SCALE METHOD

Start with the layer, then test what survives when the scale changes.

01

LOCATE

Identify the biological structure, representation, or membrane interface being studied.

02

DESCRIBE

Define the elements on each side of the interface and how they can interact.

03

MEASURE

Specify which observations are direct and which require processing, modeling, classification, or inference.

04

COMPARE

Examine variation across organisms, images, cells, environments, samples, or experimental conditions.

05

CHANGE SCALE

Ask whether conclusions remain valid when moving from local interactions to community patterns, image-level structure, or cellular function.

06

CHALLENGE

Identify uncertainty, alternative explanations, representation limits, and evidence that could contradict the interpretation.

EDUCATIONAL REFERENCE POINTS

Six researchers across ecology, biomedical imaging, and cellular electrophysiology.

These profiles are presented as educational reference points for exploring public academic work. They are not presented as members, employees, partners, collaborators, representatives, endorsers, or affiliates of Between Layers.

Platform contact note The first three email addresses are platform contact addresses supplied for this site and are not presented as verified university or institutional email accounts.

MM
COMMUNITY · ESTONIA

Mari Moora

University of Tartu · Institute of Ecology and Earth Sciences · Department of Botany

Vice Rector for Research · Professor in Community Ecology

Research on ecological interactions underlying plant-community diversity and composition, including competition and facilitation among plants, mycorrhizal symbiosis, plant-fungal relationships, fungal community composition, plant traits, biodiversity, community assembly, environmental gradients, plant invasion, and ecological patterns across local and global scales.

  • Community ecology
  • Plant-fungal interactions
  • Mycorrhiza
  • Biodiversity

ORCID 0000-0002-4819-7506

Platform contactmarimoora@cloudforlaw.org
TV
IMAGE · SLOVENIA

Tomaž Vrtovec

University of Ljubljana · Faculty of Electrical Engineering · Laboratory of Imaging Technologies

Professor

Research and teaching in biomedical imaging, medical image analysis, image and video processing, pattern recognition, quantitative analysis of anatomical structures, biomedical informatics, and computational methods for extracting clinically and scientifically useful information from images.

  • Biomedical imaging
  • Medical image analysis
  • Image processing
  • Pattern recognition

ORCID 0000-0002-6625-0307

Platform contacttomazvrtovec@cloudforlaw.org
MA
CURRENT · OMAN

Majid K. Al Salmani

Sultan Qaboos University · College of Medicine and Health Sciences · Department of Physiology

Assistant Professor

Research in cellular and molecular physiology with emphasis on ion channel physiology and pharmacology, genetically inherited ion channel diseases, cardiac arrhythmias including Long QT syndrome, cystic fibrosis, membrane electrical activity, cellular models, and the physiological consequences of altered channel function.

  • Ion channels
  • Cellular physiology
  • Long QT syndrome
  • Channelopathies

ORCID 0000-0003-1909-0141

Platform contactmajidk.alsalmani@cloudforlaw.org
JD
COMMUNITY · ESTONIA

John Davison

University of Tartu · Institute of Ecology and Earth Sciences · Plant Ecology Laboratory

Associate Professor of Plant Ecology · Doctoral tutor

Research on plant ecology, biodiversity, community assembly, plant-mycorrhizal interactions, arbuscular mycorrhizal fungi, microbial biogeography, ecological niches, high-throughput sequencing, phylogenetic information, organism traits, and quantitative methods for understanding plant and fungal communities.

  • Plant ecology
  • Mycorrhizal fungi
  • Community assembly
  • Microbial biogeography

ORCID 0000-0002-0161-6195

Educational reference point

ZS
IMAGE · SLOVENIA

Žiga Špiclin

University of Ljubljana · Faculty of Electrical Engineering · Laboratory of Imaging Technologies

Professor · Medical Image Analysis · Robot Vision

Research in medical image analysis, computer vision, image processing, biomedical image interpretation, quantitative imaging, image-derived measurements, computational methods, pattern analysis, and systems for extracting structured information from biomedical and visual data.

  • Medical image analysis
  • Computer vision
  • Biomedical imaging
  • Image processing

ORCID 0000-0001-8300-0417

Educational reference point

JH
CURRENT · UNITED KINGDOM

Jules C. Hancox

University of Bristol · Bristol Medical School

Professor of Cardiac Electrophysiology

Research on cardiac electrophysiology and membrane proteins involved in electrical activity, including ion channels, ion exchangers, cardiac action potentials, atrioventricular node physiology, electrophysiological techniques, calcium cycling, acidosis, arrhythmia mechanisms, and the cellular basis of cardiac electrical function.

  • Cardiac electrophysiology
  • Ion channels
  • Membrane currents
  • Cardiac electrical activity

ORCID 0000-0002-2055-6482

Educational reference point

REFERENCE STATUS

Academic reference does not imply participation.

Between Layers is an independent educational prototype. Academic names and institutional references are included solely to help readers discover relevant areas of public scholarship.

The first three platform contact addresses were supplied specifically for this site. They are not presented as verified personal, university, institutional, or employer-provided email accounts.

The remaining profiles are educational reference points only and are not presented as participants in, contributors to, endorsers of, or affiliates of this resource.

STUDY SHEETS

Open a layer and inspect the evidence inside it.

Browse educational notes across community ecology, mycorrhizal interactions, biomedical image analysis, ion channels, and cellular electrophysiology.

10 sheets

Community EcologyWhat makes an ecological community more than a list of species?Explore why interactions, abundance, environment, and spatial scale matter when studying biological communities.

Species composition, abundance, competition, facilitation, environmental filtering, dispersal, functional traits, spatial variation, community assembly, disturbance, and sampling all matter. The presence of the same species does not necessarily imply the same ecological community structure.

  • community ecology
  • biodiversity
  • species interactions
  • community assembly
Plant InteractionsWhen does a neighboring plant help rather than compete?Explore how plant interactions can shift between competition and facilitation.

Resource competition, light, water, nutrients, environmental stress, plant size, spatial proximity, facilitation, microclimate, life stage, density, and species traits can shift ecological interactions across environmental conditions.

  • plants
  • competition
  • facilitation
  • ecology
MycorrhizaWhat happens at the interface between a root and a mycorrhizal fungus?Explore one of the most widespread symbiotic relationships in terrestrial ecosystems.

Mycorrhizal fungi interact with plant roots, fungal networks can influence nutrient acquisition, and plants supply carbon compounds. Associations differ among species and can influence plant performance, community assembly, and ecosystem processes; they are not universally beneficial.

  • mycorrhiza
  • roots
  • fungi
  • symbiosis
Ecological ScaleWhy can an ecological pattern change when the scale changes?Explore how local observations and global distributions can reveal different patterns.

Sampling grain, spatial extent, local communities, regional species pools, biogeography, environmental gradients, species distribution, statistical aggregation, and nested scales shape conclusions. A finding at one spatial scale may not apply at another.

  • scale
  • biogeography
  • ecology
  • sampling
Biomedical ImagingWhat makes a medical image a scientific measurement?Explore why producing an image is only the beginning of quantitative analysis.

Image acquisition, resolution, contrast, noise, reconstruction, preprocessing, anatomical interpretation, measurement, calibration, observer variation, and computational processing matter. Image-derived quantities depend on both biological structure and the imaging process.

  • medical imaging
  • measurement
  • biomedical data
  • image quality
Image SegmentationWhat does it mean to segment an anatomical structure?Explore how researchers define boundaries between regions in a medical image.

Pixels or voxels, anatomical regions, manual and automatic segmentation, machine-learning approaches, boundary uncertainty, class imbalance, reference annotations, Dice-style overlap, and clinical context make segmentation an interpretive and computational task—not simply drawing a line.

  • segmentation
  • anatomy
  • medical image analysis
  • boundaries
Image RegistrationWhy do biomedical images need to be aligned?Explore how researchers compare structures acquired at different times, positions, subjects, or imaging modalities.

Rigid and non-rigid transformations, landmarks, similarity measures, coordinate systems, deformation, multimodal imaging, longitudinal studies, interpolation, and validation affect alignment. A successful visual alignment is not automatically anatomically correct.

  • registration
  • medical imaging
  • alignment
  • geometry
Ion ChannelsHow can a protein control an electrical current?Explore how membrane ion channels regulate selective movement of charged particles.

Lipid membranes, ionic gradients, channel proteins, selectivity, gating, voltage dependence, conductance, membrane potential, electrophysiological measurement, and pharmacology shape current. It depends on both the channel and surrounding electrochemical conditions.

  • ion channels
  • membrane
  • electrophysiology
  • current
Cardiac ElectrophysiologyWhy can altered potassium-channel function affect heart rhythm?Explore the relationship between ion currents and cardiac electrical repolarization.

Cardiac action potentials, potassium currents, hERG channels, repolarization, Long QT syndrome, inherited variants, drug effects, arrhythmia risk, cellular models, and electrophysiology connect molecular change to function. Clinical risk requires multiple levels of evidence.

  • hERG
  • Long QT syndrome
  • cardiac electrophysiology
  • potassium channels
Cross-Scale ReasoningCan an interface explain an entire biological system?Compare ecological interactions, image boundaries, and membrane currents without treating them as equivalent mechanisms.

An ecological interface describes relationships among organisms and environments, an image boundary belongs to a representation of biological structure, and a membrane interface is a physical cellular structure. Scale, abstraction, measurement, models, causal inference, representation, and uncertainty limit extrapolation from one layer of evidence.

  • interfaces
  • scale
  • biological systems
  • scientific reasoning

ABOUT BETWEEN LAYERS

Understanding a system often means deciding which layer to observe.

Between Layers is an independent educational prototype connecting community ecology, biomedical imaging, and cellular physiology.

It does not suggest that ecological communities, image-analysis boundaries, and membrane ion channels are equivalent biological phenomena.

Instead, it compares a shared research challenge: identifying interfaces, measuring structure and interaction, and deciding how evidence changes when researchers move between scales.

It is not a university, research institute, hospital, medical company, environmental organization, imaging company, pharmaceutical company, laboratory, professional association, or commercial service.

01

Interfaces organize interaction

Biological systems often depend on relationships across physical, ecological, or analytical boundaries.

02

Representations are selective

Biomedical images make structures visible, but the representation does not contain every property of the underlying biology.

03

Scale changes interpretation

A local interaction, image measurement, or cellular current may be important without being sufficient to explain the entire system.

CHANGE THE VIEW

Choose one layer, then ask what becomes visible from the next.

Browse study sheets, compare interfaces, and examine how ecological interactions, biomedical representations, and cellular currents require different kinds of evidence.